GENETIC ANALYSIS OF BMP-4 GENE IN BADDI GOAT BREED OF DISTRICT KHAIRPUR MIR'S SINDH PAKISTAN

Abstract

Bone morphogentic protein 4 is a protein that found in goat, cattle, and human are encoded by BMP4 gene. the candidate gene is found on chromosome 10q in goats. BMP4 gene is the subfamily of the superfamily named as transforming growth factor beta. it is an evolutionary conserved member of BMPs family. BMP4 is a protein  coding gene that concern with connective and soft tissues of the body structure . it play main role in goats, cattle, human and other animal's bodies for maintaining the body activities such as  cell proliferation, differentiation, apoptosis and migration. Present study was aimed to investigate the mutational variations found with same area of indigenous goat breed. The Genomic DNA was extracted from blood samples of Baddi goat breed. The samples were transported and stored at 4 degree for further processing of DNA extraction. The targeted region of BMP4 gene that was amplified by the specific set of primers. The amplified products were sequenced by the ABI Genetic Anayzer 3500 and sequencing data was analyzed by Bio Edit v 7.2 and blasting was performed on ensemble.org. Result revealed that about 4 missense mutations found the breed with help of PCR, gel electrophoresis and DNA sequencing. The missense mutation was identified by the replacement of genetic codon from original codon such as first missense mutation found in replacement of Serine by Threonine at 157Bp, second and third from Glycine to isolecucine at 159 Bp and 173 Bp respectively. while fourth mutational variation occured from Arginine to Serine at 177 Bp. However, the percentage of mutation in Baddi breed was greater than 1% it means there was polymorphism has been found with replacement of original amino acids with variate amino acids that play a significant role in improvement of goat characteristic such as body, muscles, milk and meat quantities. Obtained result revealed a great heterogeneity in genetic makeup of Baddi breed and hence could be used in genetic marker which assisted the selection of Baddi breed than other breeds.