HARNESSING COCONUT HUSK: SOLVENT-BASED EXTRACTION AND ITS ANTIMICROBIAL ACTIVITY AGAINST FOODBORNE PATHOGENS

Authors

  • Sana Khan College of Engineering and Technology, Ziauddin University, Karachi, Pakistan. Author
  • Syeda Bushra Zafar College of Engineering and Technology, Ziauddin University, Karachi, Pakistan. Author
  • Marium Shaikh Department of Food Science & Technology, University of Karachi, Karachi, 75270 Pakistan Author
  • Mehwish Faiz College of Engineering and Technology, Ziauddin University, Karachi, Pakistan. Author
  • Syeda Hafiza Afsheen Zafar Bahria University Health Sciences Campus, Karachi-75500, Pakistan. Author
  • Shahzad Nasim The Begum Nusrat Bhutto Women University Sukkur Author

DOI:

https://doi.org/10.71146/kjmr235

Abstract

Natural sources of antimicrobial agents have gained attention as antibiotic resistance continues to threaten global health. Coconut husk, a renewable agro-industrial byproduct rich in phenolics, flavonoids, and tannins, demonstrates antimicrobial properties. This study evaluates coconut husk extracts prepared with ethanol, methanol, and water solvents for antimicrobial activity against four food borne pathogens including Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Salmonella typhi. The ethanol extract showed significant antimicrobial activity, particularly at 48 hours, producing the largest inhibition zones (up to 29 mm for P. aeruginosa), due to its ability to extract a broader range of bioactive compounds. Methanol and water extracts showed moderate activity but were less effective compared to ethanol. Structural changes observed in microbial cell membranes after coconut husk extraction were confirmed by SEM, which indicated disruption of cellular integrity as mode of action of the antimicrobial activity. Active compounds present in phytochemical analysis confirmed to be alkaloids, tannins, flavonoids, and phenolics. The antimicrobial activity of coconut husk extracts indicating their potential to use in food preservation reducing pathogen load and extending shelf life.

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Published

2025-01-28

Issue

Section

Health Sciences

How to Cite

HARNESSING COCONUT HUSK: SOLVENT-BASED EXTRACTION AND ITS ANTIMICROBIAL ACTIVITY AGAINST FOODBORNE PATHOGENS. (2025). Kashf Journal of Multidisciplinary Research, 2(01), 128-137. https://doi.org/10.71146/kjmr235