DEVELOPMENT AND VALIDATION OF REVERSE PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (RP-HPLC) METHOD FOR ESTIMATION OF TRIAMCINOLONE ACETONIDE (KENACORT A-40) IN BULK AND ITS PHARMACEUTICAL FORMULATION

Abstract

A straightforward and precise Reverse Phase High-Performance Liquid Chromatography (RP-HPLC) for the estimation of triamcinolone Acetonide was developed. Methanol: water mixture was used as the mobile phase. The validation parameters like linearity, accuracy, precision, robustness, the Limit of detection, and the Limit of quantitation were performed for Triamcinolone Acetonide_. Concentration was found to be around 25mg/mL. Recovery and assay studies of Ranitidine HCl were 99%, indicating that the proposed method can be adopted for quality control analysis of Ranitidine HCl_. Validation of the Method for the drug and its formulation was found to be Precise, Accurate, Selective, and Sensitive. RSD was recorded up to the maximum Limit of 1.302%  within the allowed Limit of%. Accuracy/Recovery was seen as 100%, 120%.  Intermediate Precision was observed as 100%, while reproducibility was found as 100%. Co-relation Co-efficient & Y-intercept are also within the Limit of 0.9988 & 0.2512 with linear graph.