COMPARATIVE ANALYSIS OF DIFFERENT FRACTIONS AND SDS-PAGE PROTEIN PROFILES IN MUTANT AND NON MUTANT WHEAT (TRITICUM AESTIVUM L.)VARIETIES

Abstract

In Pakistan different wheat varieties are grown by mutation breeding, or cross breeding, the most important breeding methods successfully used in domestic wheat to obtain new lines of mutant, cross breed or hybrid varieties which have better agronomic values. The current study was aimed to evaluate the mutation induced changes in different mutant varieties, cross breed and hybrid variety present in Sindh, Pakistan. Comparative study of 30 (10 mutant, 10 cross breed and 10 hybrid) wheat cultivars collected from NIA, (Nuclear Institute of Agriculture Tandojam) was conducted. Total protein, fractions of proteins and SDS- PAGE profiling of protein” were carried out at the laboratories of Institute of Biochemistry University of Sindh Jamshoro. All experiment were performed by applying standard analytical methods and data were examined using the T test and one-way analysis of variance (single factor) ANOVA to determine whether a value was significant (P < 0.05) or very significant (P < 0.001). The findings showed that the application of induced mutation has a great impact on varieties development and their properties which are significantly differed. The Albumin content exhibited significant differences among varieties while the total protein constituent observed constant. These finding suggests that mutation breeding and genetic background of crossbreed or hybrid varieties may influence nutrient profile of wheat varieties. However, the data of this research study will be an important resource, in breeding strategies for growing wheat varieties with desired composition, nutritional and functional properties.